Genetic variation of DNA testing of local vine varieties from Bulgaria and Greece

A unique DNA study of local local grapevine varieties from Bulgaria and Greece was performed as part of the "SOS project for endangered traditional vine varieties".
The aim of this study was to estimate the genetic diversity of eight Greek and nine Bulgarian local grapevine varieties with the use of seven microsatellite markers. Statistical analysis of data showed that there is high degree of genetic heterogeneity among most of the varieties studied, as well as a close genetic relationship in three variety pairs. Based on our results, we suggest the synonymy of Greek Pamid & Bulgarian Pamid and Greek Zoumiatiko & Bulgarian Dimyat. On the other hand, Greek Keratsuda and Bulgarian Keratsuda varieties should be characterized as homonyms. Additional molecular work is needed for a thorough analysis of Greek and Bulgarian grapevine genepool.

The aim of this study was to analyze the genetic diversity and differentiation of various local Greek and Bulgarian grapevine varieties, using microsatellite genetic markers. Besides that, our goal was also to clarify the putative relationship of varieties coming from the above two countries and characterized as common.

Results and Discussion. Genetic variation

In total, 53 different alleles were found in the 384 samples analyzed with seven microsatellite loci. Polymorphism of the markers was low to medium, ranging from four (VVMD25) to ten (VrZAG79) alleles. Mishej Sandanski variety exhibited the highest mean number of alleles (4.71), while the lowest respective value was found in Keratsuda(G) variety (1.57). The population Ho values ranged from 0.57 to 1 with a mean of 0.79, while the respective range for He was much lower (0.3 to 0.6) and averaged 0.45, indicating a heterozygote excess in our data. Regarding the mean value for Ho (0.79) found herein, this was similar to Ho values reported in other studies analyzing grapevine cultivars with microsatellite markers (see Introduction for related references).
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